Restriction Digest of Plasmid DNA

Multiple Choice Questions on Restriction enzymes

Refer to the plasmid map below. Note the total size and the locations of cutting sites for different restrictionendonucleases.0 bp500 bp EcoRIHow many fragments, and what sizes, wouldyou expect to see if you cut with the followingcombinations of enzymes in a restriction digest:1. EcoRI only:-900 bp BamHI3000 bpXholPlasmid Size4000 bp2. BamHI only:3. BamHI and Xhol:4. EcoRI and BamHI:1700 bp EcoRIFor each of the numbered reactions above, draw the expected bands in the corresponding lanes on the g

[GET ANSWER] 7: 56 ⋯Δ(1) 4#1 Mol Bio Re...Complete the following problems.Restriction enzymes (REs), which cut D NA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, pMBBS, through restriction digests. You subjected the pMBBS plasmid to complete digestion by